產(chǎn)品說明

中文名稱: 阿非迪霉素 ≥98%

英文名稱: Aphidicolin

別名: 艾菲地可寧  (3R,4R,4aR,6aS,8R,9R,11aS,11bS)-4,9-bis(hydroxymethyl)-4,11b-dimethyltetradecahydro-8,11a-methanocyclohepta[a]naphthalene-3,9-diol

CAS : 38966-21-1

分子式: C₂₀H₃₄O₄

分子量: 338.48

產(chǎn)品簡介：Aphidicolin 是 DNA 聚合酶 α 和 δ 抑制劑，通過干擾 DNA 聚合酶的活性來阻止細(xì)胞有絲分裂。Aphidicolin 是一種來源于 Cephalosporium aphidicola 的抗生素, 能夠抑制細(xì)胞脫氧核糖核酸合成和單純皰疹病毒生長。Aphidicolin 具有抗正痘病毒活性，能夠增強(qiáng)阿拉伯糖基核苷對細(xì)胞凋亡 (apoptosis) 的誘導(dǎo)作用。

溶解性：溶于DMSO(50mg/ml)和甲醇(10mg/mL)

儲備液保存：-80°C, 6 months   -20°C, 1 month

體外研究：Aphidicolin (0.5 μM, 5 μM; 0-5 d) selectively kills neuroblastoma cells, but shows moderate cytotoxicity on normal human embryonal cells and HeLa, H9, A549 and Caco-2 cell lines.

Aphidicolin (0.4 μg/mL; 3 d) arrests cell cycle at G2 phase. Aphidicolin (100 nM-10 μM; 48 h) inhibits cell proliferation via the p53-GADD45β pathway and (1 μM; 24 h) induces apoptosis in AtT-20 cells.

Aphidicolin (10 μM; 0-6 h) decreases the phosphorylation of Akt, (100 nM-10 μM; 24 h) increases the mRNA levels of the stress response gene growth arrest and DNA damage-inducible 45β (GADD45β), a putative downstream target of p53.

Aphidicolin (10 μM; 0-6 h) inhibits Varicella-zoster virus (VZV) with EC50s of 0.5-0.6 μM, with low cytotoxicity.

體內(nèi)研究：Aphidicolin, has been developed with higher solubility agent, namely Aphidicolin glycinate (AG; NSC 303812).

Aphidicolin glycinate (100 mg/kg; i.p.; once every 3 h; 9 d) shows anti-tumor activity against the implanted B16 melanoma and M5076 sarcoma in murine, producing maximum increased life spans of 75%.

保存條件：-20℃

注意事項(xiàng)：

            1、為了您的安全和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。

            2、以上信息僅做參考交流之用。

包裝規(guī)格：1mg   5mg   10mg

特點(diǎn)： 性價比高，品質(zhì)優(yōu)越

適用范圍：

保存： -20℃